Composite

Part:BBa_K2581010

Designed by: Laura Sans Comerma   Group: iGEM18_UPF_CRG_Barcelona   (2018-10-09)


FadL gene with ATC inducible promoter with weak RBS.

The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own E.Coli outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation across the cell membrane via a transportation/acyl-activation mechanism. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the fadL gene (outer membrane transporter via a acyl-activation mechanism) obtained of the own E.Coli DH5-alpha strain genome (BBa_K2581015, being a twin of BBa_k861500, a part not available).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1286
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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